摘要
用提纯的Ａｂ２制成免疫制剂，在大白鼠、小白鼠可诱导特异性ＶＳＧ抗体（Ａｂ３）。免疫保护试验结果表明，Ａｂ２制剂免疫动物对攻虫感染具有明显的保护效应。单独用抗独特型抗体免疫的小白鼠、大白鼠、豚鼠对５０条同型锥虫攻击的保护率分别为６０％，６０～８０％，８０％；抗独特型抗体作为“引子”，可使小白鼠获得８０～１００％的保护，对大白鼠和豚鼠的保护率为１００％。单独使用抗独特型抗体免疫的小白鼠对５个数量（２５，５０，１００，２００，４００）锥虫攻击的保护率分别为８０％，６０％，２０％，０％，０％，而作为“引子”免疫小白鼠对５个数量锥虫攻击的保护率分别为１００％，８０％，４０％，２０％，０％。这些结果表明，制备的兔抗独特型抗体（Ａｂ２）含有ＶＳＧ表位的内影像，具有模拟ＶＳＧ的功能，在实验动物体内可诱导主动免疫，因此可作为一种潜在的疫苗制剂为家畜伊氏锥虫病的免疫预防提供一个可能的途径。
Rabbit anti-Id(Ab2 ) preparation could elicit specific anti-VSG antibodies(Ab3)in rats andmice which could bind to VSG,as detected by indirect ELISA. Ab2 preparation induced significant protectiveimmunity against the challenge infection in animals.Two vaccinations of Ab2 alone resulted in protectionrates of 60%,60～80%,80% respectively to a challange of 50 trypanosomes in mice,rats and guinea pigs;One vaccination of Ab2 as a priming showed protection rates of 80～100%,100%, 100%respectively to achallange of 50 trypanosomes in mice,rats and guinea pigs. Two vaccinations of Ab2 alone induced protec-tion rates of 80%,60%,20%,0%,0%respectively to challanges of 25,50,100,200,400 trypanosomes inmice; One vaccination of Ab2 as a priming showed protection rates of 100%, 80%,40%, 20%,0%respec-tively to challanges of 25,50,100,200,400 trypanosomes in mice. These observations indicated that rabbitanti-idiotypic antibody(Ab2)embodied internal images of VSG epitopes and functioned as surrogate ofantigens in vivo for inducing active immunity in animals, suggesting a possible aIternative approach for im-munological vaccination of animals against trypanosomiasis as a potential vaccine preparation.
出处
《中国兽医杂志》
CAS
北大核心
1995年第3期3-5,共3页
Chinese Journal of Veterinary Medicine
