摘要
研究了人淋巴因子ｒｈＩＬ－４、ｒｈＩＬ－２、ｒｈＩＦＮ－γ对ＰＭＡ活化的扁桃体Ｂ淋巴细胞ＣＤ２３分子表达的调节作用。实验结果表明：１．ｒｈＩＬ－４能够显著增加ＰＭＡ作用下的Ｂ细胞ＣＤ２３表达，呈剂量依赖性反应；ｒｈＩＦＮ－γ对活化后Ｂ细胞ＣＤ２３表达呈明显抑制作用；ｒｈＩＬ－２对ＣＤ２３表达的抑制作用微弱。２．在ＰＭＡ单独刺激下Ｂ淋巴细胞ＣＤ２３的表达在第３天达高峰，而ＰＭＡ与ＩＬ－４共同作用下ＩＬ－４能够持续促进ＰＭＡ作用下的扁桃体Ｂ细胞ＣＤ２３的高表达，在作用的第５天ＣＤ２３阳性率可高达７５．４％。由此可见Ｂ细胞ＣＤ２３分子的表达受到Ｔ细胞源性淋巴因子的调控，这种调控对于免疫应答的调节可能具有重要意义。此外对Ｂ淋巴细胞经ＰＭＡ及淋巴因子活化后，反映细胞分裂状态的￣３Ｈ－ＴｄＲ、￣３Ｈ－ＵＲ掺入ｃｐｍ值与ＣＤ２３表达的相关性进行探索。发现两者无明显相关性。
The effects of T cell derived lymphokine rhIL-4,
rhIL-2 and rhIFN-γ on CD23 expressionon PMA activated B cell were
analysed. The results showed that rhIL-4 could obviously elevatedCD23
expression. The optimum conceneration of rhIL-4 was at 50～
100U/ml.Unlike rhIL-4,rhIFN-γ significantly reduced CD23 expression.
rhIL-2 could also suppress the CD23 expres-sion,but the effect was
much weaker compare with rhIFN-γ. The time effect on CD23
expres-sion on B cell stimulated bv PMA or PMA+IL-4 were also
analysed. The results show thatCD23 expression on B cell actived by
PMA reached the maximum(44.4%)on the third day.otherwise,CD23 express
on B cells actived by PMA+rhIL-4 steadily increased during 5
daysobservation and the percentage of CD23 positive cells fifth day
reached 75.4%。 In addition,weobserved the relatiohship between the
percentage of CD23￣(+) B cells and￣3H-rdR & ￣3H-UR incor-poration
in activated B cells, The results showed that the change of
incorporation value(￣3H-TdR & ￣3H-UR) did not cause responsably the
change of CD23 expression on actived B cell.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1995年第1期6-9,共4页
Chinese Journal of Immunology
