摘要
卡那霉素链霉菌温和性噬菌体ＳＫＪ１ＤＮＡ上有２个ＡｖａⅠ和ＳｍａⅠ酶切位点，８个ＳａｌⅠ位点，７个ＥｃｏＲⅠ位点及１２个ＢａｍＨⅠ位点。经酶切后用１％琼脂糖凝胶电泳测定ＳＫＪ１ＤＮＡ大小为６０．７ｋｂ．用单酶切、双酶切及片段酶切分析，得到ＳＫＪ１ＤＮＡ的ＡｖａⅠ、ＳｍａⅠ、ＥｃｏＲⅠ、ＳａｌⅠ的酶切图谱及ＢａｍＨⅠ的部份酶切图谱。证明ＡｖａⅠ和ＳｍａⅠ在ＳＫＪ１ＤＮＡ上具有相同酶切位点。并经过酶切结果分析和图谱构建，ＳＫＪ１ＤＮＡ可能为１个环状分子。
Actinophage SKJ1 DNA has 2 cut sites for AvaⅠand SmaⅠ,8 for SalⅠ,7 for EcoRⅠand 12 for BamHⅠ.The size of SKJ1 DNA was estimated to be about 60.7 kbby means of electrophoresis on 1%agarose gel digested with various enzymes. With single,double enzyme digestiom and fragment isolation,the restriction map of SKJ1 DNA for AvaⅠ,SmaⅠ, EcoRⅠ,SalⅠand a partial restriction map for BamH Ⅰ have been constructed.Ithas shown that AvaⅠaidSma Ⅰ have the same cut sites on SKJ1 DNA. Study on theenzyme digestion and the construction of the restriction map suggested SKJ1 DNA to be acircular molecule.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
1995年第5期327-332,共6页
Chinese Journal of Antibiotics
基金
"七五"攻关项目
