摘要
茯苓素在小鼠体内诱生肿瘤坏死因子的机制在于增强ＴＮＦ基因的转录。实验结果表明：莅菩素以２７～８１ｍｇ／ｋｇ剂量刺激小鼠巨噬细胞，可得到较高效率的ｍＲＮＡ表达，超过此剂量可由于药物的毒性作用而造成基因转录水平的下降。ｍＲＮＡ的杂交结果（ＳｌｏｔＢｌｏｔｔｉｎｇ）可通过Ｔｕｂｕｌｉｎ探针的杂交而得到验证。
The mechanism of stimulation of TNF production by macrophages causedby poriatin was related to enhancement of transcription of TNF gene.Experiments showedthat higher effcient expression of TNF mRNA can be obtained with poriatin at doses of 27～81mg /kg,Over the optimum dosage,the level of transcription of TNF mRNA was loweredand it might be due to the toxicity of the drug.The result of slot blotting was identified by rehybridization of α-tubulin probe.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
1995年第1期31-34,共4页
Chinese Journal of Antibiotics
基金
医科院院基金
关键词
茯苓素
肿瘤坏死因子
巨噬细胞
杂交
中草药
Poriatin
Tumor necrosis factor
Macrophage
Hybridization
Autoradiography
