摘要
应用全细胞膜片钳制技术观察了牛磷酸对豚鼠单一心室肌细胞慢内向钙离子电流(Ica)的作用。实验结果显示:牛磺酸能减少心室肌细胞的Ca^(2+)内流。此作用具有明显的电压依赖性,对其反转电位无明显影响。牛磺酸减少Ica的作用也具有剂量依赖关系,0.1μmol·L^(-1)牛磺酸减小Ica约17％,最大效应浓度接近100μmol·L^(-1)。在停止刺激休息后,首次给予刺激,10μmol·L^(-1)牛磺酸则使Ica减小47％。通过增加刺激频率,牛磺酸能促进Ica的减小。上述结果表明,牛磺酸以电压和使用依赖的方式减少心室肌细胞的钙离子内流。
With patch clamp method,the effects of taurine (Tau) on slow inward calcium current (Ica) were investigated in single ventricular cells of guinea pigs. Tau decreased Ica in a dose- and voltage-dependent mode,but had little effect on reversal potential. Tau 0. 1 umol - L-1 decreased Ica by 17% and its maximal effective concentration was near 100 umol -L '. The initial block of Tau had already reached by 47% after a rest out of stimulation. Tau facilitated the reduction of lea through increasing clamp pulse frequency. The obtained results suggested that it decreased Ica in a use-dependent manner.
出处
《中国海洋药物》
CAS
CSCD
1995年第4期1-5,共5页
Chinese Journal of Marine Drugs
关键词
牛磺酸
钙离子
电流
钙通道阻滞剂
膜片钳
Taurine (Tau )
calcium current (Iea )
calcium channel blockade
patch clamp method
