摘要
本实验采用球囊剥脱术在体损伤血管，于术后３天和１０天取出血管做平滑肌细胞培养。结果发现，血管损伤后血管平滑肌细胞增殖活性显著增加，ＤＮＡ和蛋白质合成加速，细胞数目增多。在血管平滑肌细胞增殖过程中，钙内流增加，胞内钙含量升高。应用１０μｍｏｌ·Ｌ￣（－１）异搏定不但对钙稳态变化有一定的抑制作用，而且还对血管损伤诱导的平滑肌细胞增殖有抑制作用。这些结果提示血管损伤可致血管平滑肌细胞增殖；钙稳态失衡可能是血管损伤诱导血管平滑肌细胞增殖的细胞学机制之一。
Vascular smooth muscle cells (VSMC) cultured from the rat aorta at 3 and 10 days after balloon denudation. Results showed that activity of VSMC proliferation increased significantly, synthesis of DNA and protein accelerated, and cell count increased. During VSMC proliferation, calcium influx increased, calcium content elevated. 10 μmol·L￣(-1) Verapamil inhibited not only the changes of calcium homeostasis, but also vascular injury induced SMC proliferation. These findings indicated that vascular injury induced VSMC proliferation. Disturbances of calcium homeostassis might be one of cellular mechanisms of vascular injury induced VSMC proliferation.
出处
《中国动脉硬化杂志》
CAS
CSCD
1995年第3期212-215,共4页
Chinese Journal of Arteriosclerosis
关键词
血管损伤
平滑肌细胞增殖
钙稳态
Vascular injury
Vascular smooth muscle cell proliferation
Calcium homeostasis
