摘要
本文报道人疱疹病豢－６型（ＨＨＶ－６）ｐＳＴＹ２８ＤＮＡ片段的序列测定。应用分子克隆、缺损突变体（Ｄｃｌｅｔｉｏｎｍｕｔａｎｔ）制备和序列测定等技术，完成了３．９ｋｂＨＨＶ－６ｐＳＴＹ２８ＤＮＡ片段的全序列测定。经ＤＮＡＳＩＳ核酸蛋白软件分析，该片段含有两个开读框架（ＯＲＦ）核糖核苷酸还原酶（ＲＩＲ）ＯＲＦ有２４１４个核苷酸，可编码８０５个氨基酸；Ｐ４１蛋白由１１００个核苷酸组成。与其他疱疹病毒作氨基酸同源性比较，ＨＨＶ－６ＲｉＲ与人巨细胞病毒（ＨＣＭＶ）有高度同源性，最适记分（Ｏｐｔｉｍｉｚｅｄｓｃｏｒｅ）达４５９。实验结果支持Ｅｓｆｔａｔｈｉｏｕ提出的论点，ＨＨＶ－６属于β－疱疹病毒。
hs report confirms the sequence of pSTy 28 fragment of HHV-6.We jave determined thecomplete nucleotide sequence,The sequence data have revealed open reading frame(ORF)of 2414 nucleotides encoding a ribonucleotide reductase (RiR) protein of 805 amino acids and 1100nucleotides encoding a P41 protein,To examine the relatedness of RiR protein with those of otherherpesvirus,we compared their amino acid Sequence in pairwise combination,Interestingly,RiRORF of HHV-6 has shown to have the highest amino acid humology with the RiR of HCMV.The optimized score reaches 475.Biochemical analvsis of RiR has ind icated the active site ofthioredoxin ontaining two cysteins separated by two amino acids.This sequence was found onlyonce in the E.coli,HSV-2,EBV and mouse RiR protein,but we did not find it in the HHV-6and HCMV RiR protein.Those data support Esftathiou′s view that HHV-6 belongs to betaherpes viruses.
出处
《中国病毒学》
CSCD
1995年第4期309-316,共8页
Virologica Sinica
