摘要
木毒蛾质型多角体病毒呈不规则多面体，大小差异较大，大多数在０．８～２．１μｍ之间；病毒粒子在多角体内随机分布，病毒粒子为正二十面体结构，有１２个顶体，每个顶体上均有一个球状结构，平均直径约为６０ｎｍ。经ＳＤＳ－ＰＡＧＥ分析，多角体蛋白由一条分子量为３ｌｋＤ的主带及３条次带组成；病毒粒子结构蛋白由６条多肽组成，分子量在１２．６～３３ｋＤ之间。多用体蛋白富含天冬氨酸，谷氨酸及酪氨酸，而半胱氨酸和甲巯氨酸含量较少，其碱性氨基酸与酸性氨基酸之比为１．４３。病毒核酸对ＲＮａｓｃ和ＤＮａｓｅ的稳定性试验及对甲醛的稳定性试验和吖啶橙染色试验，证明孩核酸为双链ＲＮＡ，其解链温度为８７℃，由１０个基因片段组成，分子量在２．４６×１０６～０．３５×１０６道尔顿之间，总分子量为１４．４２×１０６道尔顿，电镜分析研究显示该核酸长度在０．５μｍ、１．０μｍ和１．３μｍ处各有一个分布峰。
he Lymantria,xylina Swinhoe cytoplasmic polyhedra xhows a quite difference ranging from 0.8 to2.lμm in diameter.The virions were randomly distributed in a polyhedron;The virions show icosahedron, and about 60 nm in diameter with l2 spikes.By the analysis of SDS-PAG E,the polyhedral pro-teins contain one major band(31000 Dalton)and three minor tsands.The virlon has 6 polypoptidesranging from 12600 to 33000 Daltons,The protein from LxCPV is rich in aslxirtic acid,glutamic acidand tyrosine,but poorer in cystein and methionine; The ratio of basic amino to acidic amino is l.43.By the stability test of the LxCPV RNA to RNase ,DNase and formaldehyde,the staining reaction ofacridine orange,the RNA is demontrated as double-strand RNA(dsRNA)with a Tm of 87℃.TheRNA genome consists of ten molecular mass from 2.46 to 0.35 MD,and with a totol estimated moleeular mass of l4.42 MD,Three distributive peaks of LxCPV RNA segments contour length are foundat 0.5μm,1.0μm and l.3μm respectlvely by electron micrcacopic analysis.
出处
《中国病毒学》
CSCD
1995年第1期67-74,共8页
Virologica Sinica
关键词
木毒蛾
质型
多角体病毒
形态结构
理化特性
Lymantria xylina Swinhoe, Cytoplasmic polyhedosis virus (CPV),Fine structure,Biophysi cal and biochemical characteristics
