摘要
首次报道用原生质体融合技术对茯苓作生物工程育种，取得融合菌种，在空徽霍山县境内进行野外栽培试验的结果．亲本P1、p578经茯苓菌丝体原生质体分离、融合、标记和融合子检出、培养和鉴定等步骤后，制成栽培种F1、与P1、P578同时进行了对比栽培。试验结果：P1均产为0．5kg／窖，P578为2．20kg／窖；F1为1．73kg／窖；F1产量介于2个亲本之间，产量不够理想，对其原因作了初步分析。
Rcsult of field cultivation test by protoplastic fusion technique for biological cngln ering breeding of Poria cocos in Huoshan county, Anhui Province was reported for the first time. A fusion strain F1 was obtained from parents P1, P6578 by separation, fusion, marker of hypha protoplast and identification of recombinat. It was cultivated simultaneously with P1, P5778 for comparison. Results showed that the average cellal outputs were 1.73, 0.5 and 2.20kg respectively.As the output of strain F1 is intermediate between its parent P1 and P578, the result is considered to be unsatisfactory. The reason for such result was discussed.
出处
《中草药》
CAS
CSCD
北大核心
1995年第5期261-262,共2页
Chinese Traditional and Herbal Drugs
