摘要
用反相HPLC法测定了月腺大戟根中抗结核活性成分狼毒甲素，狼毒乙素的含量。实验采用ODS柱，以甲醇－水－10％乙酸（45：55：2）（10％氨水调pH至5．4）为流动相，延胡索乙素为内标，280nm为检测波长，可在30min内完成测定。本法快速，简便，灵敏性好，可用于原药材的质量控制。
An HPLC method for the quantitative analysis of bis (5-formyl-furfuryl ) -ether an d 2, 4-dihydroxy-6- methoxy-3-methyl- acetophenone in Chinese drug,Langduc (Euphorbia ebracteolata)was developed. The solvent system used was methanol-water-10% acid (45 : 55 : 2 ) (adjusted to pH 5.4 by 10% ammonia ) on ODS columm at 289nm.Tetrahydro palmatine was used as an internal standard. The operation can be completed in 30min.This method is sensitive,simple and accurate with good reproducibility.It can be applied to the quality control of the crude drug of E. ebracteolata.
出处
《中草药》
CAS
CSCD
北大核心
1995年第2期66-67,75,共3页
Chinese Traditional and Herbal Drugs
