摘要
以硝酸纤维素膜(NCM)为固相载体,应用间接ELISA法对纯化的马铃薯卷叶病毒(PLRV)、感染PLRV的马铃薯植株的茎、叶、休眠块茎顶端、脐部、块茎打破休眠后萌发的芽,以及饲毒后的桃蚜体内的PLRV分别进行了测定。结果表明检测纯病毒的灵敏度为45.83pg～4.583pg,测定茎、叶、休眠块茎顶端、脐部、芽的稀释度分别可达到1/2048、1/512、1/2048～1/8192、1/512～1/2048和1/2048～1/8192,和常规DAS-ELISA相比,检测的灵敏度提高至少8倍。应用Dot-ELISA至少可检测1/2头带毒蚜虫体内的PLRV。
Purified PLRV, stems and leaves of potato plant, rose-end and heel-end of dormant tuber, sprouts of broken dormant tuber with secondary infection by PLRV and viruliferous Myzus persicae adults were detected by indirect enzyme linked immunosorbent assay (ELISA) on nitrocellulose membranes respectively. The results indicated that the minimum amount of purified PLRV as little as 45. 83pg^4. 583pg can be detected and PLRV can be detected in stems and leaves of infected potato plant, rose-ends and heel-ends of dormant tuber with secondary infection,sprouts of broken dormant tuber by Dot-ELISA at dilutions of 1/2048,1/512,1/2048-1/8192,1/512-1/2048 and 1/2048-1/8192 respectively.
PLRV in viruliferous Myzus persicae adults can also be detected at least with dilution of 1/2.
出处
《植物病理学报》
CAS
CSCD
北大核心
1995年第1期61-64,共4页
Acta Phytopathologica Sinica
基金
国际马铃薯中心资助~~
