摘要
四氯化碳（ＣＣｌ＿４）与肝微粒体共同温浴，引起微粒体的丙二醛（ＭＤＡ）升高、蛋白巯基的降低和钙泵的抑制。与对照组比较具有显著性意义，且存在着剂量－反应关系及时间－反应关系。在本实验条件下，发现ＣＣｌ＿４引起蛋白巯基的降低和钙泵的抑制呈明显的相关关系。用巯基供剂ＤＴＴ、过氧化物自由基清除剂ＳＯＤ均能部分地桔抗ＣＣｌ＿４引起肝微粒休脂质过氧化（ＬＰＯ），从而保护了微粒体的蛋白硫基及钙泵。表现为ＤＴＴ＋ＣＣｌ＿４及ＳＯＤ＋ＣＣｌ＿４与单纯的ＣＣｌ＿４组比较ＭＤＡ降低、蛋白疏基升高和钙泵活性升高，且差别具有高度显著性。说明ＣＣｌ＿４所致蛋白巯基降低、钙泵抑制与ＬＰＯ有关。在无脂质过氧化的条件下，蛋白巯基络合剂Ｎ－乙酰基马来酰亚胺（ＮＥＭ）络合８０％以上蛋白巯基时，钙泵的活性也降低。说明钙泵的活性下降的机理可能是ＣＣｌ＿４先经微粒体Ｐ４５０裂解，产生自由基，引发ＬＰＯ，消耗蛋白巯基，继而引起钙泵的活性降低。
at
liver microsomes incubated with carbon te-trachlorid(CCl_4 ) resulted
in increase of malonvl-dialedehyde and decrease of protein sulfhydryl
groupcontent and the activity of the calcium pump, Thechanges showed
time-effect and dose-effect rela-tionship respectively and with
significant differencesfrom that of control. The incubation of the
micro-somes with CCl_4 and dithiothreitol(DTT) or supero-xide
dismutase(SOD)simultaneously showed thatDTT and SOD could antagonize
liper peroxidation(LPO)and restore the protein sulhydryl group
andcalcium pump. In case of absence of LPO , more than80% of protein
sulfhydryl group was specifically com-plexed by N-ethymaleimide(NEM),
and the calciumpump was also inhibited. The results suggested thatthe
calcium pump inhibited by CCI4 was associatedwith the reduction of
protein sulfhydryl group due toLPO.
出处
《职业医学》
1995年第6期2-3,共2页
China Occupational Medicine
关键词
四氯化碳
蛋白巯基
钙泵
过氧化脂质
carbon tetrachloride,
liper peroxida-tion , protein sulfhydryl group,calcium pump
