摘要
通过原生质体融合技术将二倍体酿酒酵母（Ｓａｃｃｈａｒｏｍｙｃｅｓｃｅｒｅｖｉｓｉａｅｖａｒ．ｅｌｌｏｐｓｉｏｄｅｕｓ）与单倍体糖化酵母（Ｓａｃｃｈａｒｏｍｙｃｅｓｄｉａｓｔａｔｉｃｕｓ）构建成遗传上稳定的种间三倍体融合杂种。实验结果表明，这种融合杂种象有性杂种一样能诱导产孢；对其完整和非完整四分子的遗传分析，证明了通过遗传标记互补选择法获得的种间三倍体融合杂种ＨＵ－ＫＤＦ－２４０在产孢过程中，标记基因发生了分离和交换，出现了亲二型和重组类型；四分子对可溶性淀粉的发酵和不发酵分离比为１∶２，而原养型与营养缺陷型的分离比是１∶１。
A genetically stable interspecific triploid fusion-hybrid between a diploid Saccharomyces cerevisiae var.ellopsoideus and a haploid Saccharomyces diastaticus was constracted by protoplast fusion technique.Experimental results indicate that such hybrid can be induced to sporulate as a sexual hybrid.Genetic analysis of the complete and non-complete tetrads demonstrates that the prototrophic interspecific triploid fusion-hybrid HU-KDF-240 selected by nutritional complementation experienced segregation and exchange for the genetic markers,and showed parental ditype and recombination in the meiotic process of sporulation. The results of the tetrad analysis of the hybrid HU-KDF-240 showed a 1∶2 segregation ratio for soluble starch fermenting and non-fermenting characters and a 1∶1 segregation ratio for genetic markers of the auxotrophic character in 349 complete and non-complete tetrads from 186 asci of the hybrid.
基金
国家自然科学基金
