摘要
用高效液相色谱法对５－乙基－５－对氟苯甲酰丙基巴比妥酸在家兔体内的代谢物进行了分离，用二极管阵列检测器测定了原药及代谢物紫外图谱，得出代谢物的紫外吸收性质；同时测试了原药及代谢物的质谱，推测出代谢物的可能结构，并用化学方法合成了代谢物对照品，通过核磁和质谱的测定进一步确证了结构。
The metabolite of 5-ethyl-5-(
p-fluorobenzoylpropy)harbituric acid(coded asF-B-1) in rabbit has
been separated with HPLC. UV-spectra of F-B-1 and its metabolite
obtained withDAD, in combination with EI-MS and CI-MS of F-B-1, its
metabolite and their derivatives wereexamined. Possible structure of
the metabolite was obtained.Possible metabolic way of F-B-1 is
thereduction of carbonyl on the benzene ring with formation of
hydroxyl group. This was furtherconfirmed by identity of HPLC and MS
behavior of the metabolite and NaBH4 reductive preduct. Inthe
structure of the latter, the carbonyl on the benzene ring was
reduced, as proved by NMR(lH-NMR and 13C-NMR)spectra.
出处
《药学学报》
CAS
CSCD
北大核心
1995年第8期610-614,共5页
Acta Pharmaceutica Sinica
关键词
巴比妥酸
代谢物
高效液相色谱
尿药浓度
Ethyl-5-( p-fluorobenzoylpropyl ) barbituric acid
Metabolite
