摘要
介绍了一种测定人血清盐酸劳卡尼（ｌｏｒｃａｉｎｉｄｅｈｙｄｒｏｃｈｌｏｒｉｄｅ）浓度的高效液相色谱法（ＨＰＬＣ）。不锈钢分析柱（２００ｍｍ×５ｍｍＩＤ）固定相为ＹＷＧＣ１８Ｈ３７，５μｍ颗粒。流动相为甲醇－水－０．６２５ｍｏｌ·Ｌ－１醋酸铵（８６：１３：１ｖ／ｖ），用浓氨水调至ｐＨ８．０。流速１ｍｌ·ｍｉｎ－１。取地尔硫（ｄｉｌｔｉａｚｅｍ）为内标物。紫外检测波长２２６ｎｍ。低、中、高３种浓度方法回收率分别为９５．８５％，１００．６３％，１００．０９％，由低至高四种浓度日内、日间ＲＳＤ小于７％。血清最低检测浓度为５μｇ·Ｌ－１。在２０～８００μｇ·Ｌ－１浓度范围内线性良好，ｒ＝０．９９９６。
An analytical method
was established for determination of lorcainide hydrochloride
concentration in serum using high performance liquid
chromatography(HPL.The stainless steel column was 200 mm × 5. 0 mm
ID, packed with YWG C18H37,5μm, The mobile phase consisted of
methanol-water-0. 625 mol·L-1 ammonium acetate.(86:13:1v/v),adjusted
to the pH 8. 0 with ammonium hydroxide. The flow rate was 1 ml·
min-1, using diltiazem as internal standard.Chromatography was
performed with ultraviolet detector at 226 nm. The recoveries of
lorcainide hydrochloride at 40,200 and 400μg,L-1 were 95.85%,
100.63%and 100.09%, respectively.The within-day and day-to-day RSD of
the determinations at concentrations of 100,260,400 and 800μg·L-1
were less than 7%. Linear calibration curve for lorcainide
hydrochloride was measured within the range of 20 to 800μg·L-1 with
correlation coefficient of 0.9996. The detection limit was 5μg·L-1
in serum. The HPLC method described is suitable for clinical
monitoring and pharmacokinetic study of lorcainide hydrochloride.
出处
《药学学报》
CAS
CSCD
北大核心
1995年第8期605-609,共5页
Acta Pharmaceutica Sinica
关键词
盐酸劳卡尼
高效液相色谱
血药浓度
Lorcainide hydrochloride
HPLC
Serum drug concentration
