摘要
酸枣微繁研究，成功地解决了消毒、繁殖、生根及移栽等关键技术问题。二次升汞灭菌法Ⅱ，材料污染率仅１８．１％，比一次升汞灭菌法１效果提高４．２倍。不同发育阶段适宜培养基组成（ｍｇ／Ｌ）：促进萌芽生长培养基有ＭＳ＋ＢＡ０．５ｍｇ／Ｌ＋ＮＡＡ０．５ｍｇ／Ｌ和ＭＳ＋ＢＡ３．０ｍｇ／Ｌ＋ＩＢＡ１．０ｍｇ／Ｌ二种；继代繁殖培养基：ＭＳ＋ＢＡ０．１—０．５ｍｇ／Ｌ、＋ＮＡＡ０．１ｍｇ／Ｌ培养４０天，平均苗高４．６４—３．８２ｃｍ，芽繁殖倍数为７．６—６．３；生根培养基为１／２ＭＳ＋ＩＢＡ１．０ｍｇ／Ｌ＋ＩＡＡ１．０ｍｇ／Ｌ，最高生根率为１００％。试管苗移栽成活率９７．９％，定植成活率１００％。近三年研究一个简易、快速、实用酸枣工厂化育苗生产程序已基本建成。
This experiment has solved basically some key problems of the technology of the clonal porp-agation and the factory culture seedling of wild jujube :(1)The new way of the high efficient dis-infecion that is HgCl_2 disinfecion twice,the frequency of the meterial polluted was only 18.1%,the effect of this way has rasied more four times than the way of Hg Cl_2 disinfecion one time;(2)The axillary bud and grow the medium: MS+BA0.5mg/L+NAA 0.5mg/L; MS +BA3. 0mg/L+IBA1. 0mg/L;(3)The medium of the succssion porpagate:MS+BA0.1 0. 5mg/L+NAA0.1mg/L. No.of proliferation times of buds was 7.6 6. 3,after 40 days;(4)The medi-um of growing root:1/2MS+IBA1.0mg/L+IAA1. 0mg/L,The concentration of sucrose of ev-ery medium is 2%.The lilving rate of the transplantation of the tube plant was 97.9%.The liv-ing rate of field planting was 100%.A set simple practial and quick production process of factory culture seedling of tuble。seedling has proliminary established.
出处
《西北植物学报》
CAS
CSCD
北大核心
1995年第4期301-306,共6页
Acta Botanica Boreali-Occidentalia Sinica
关键词
酸枣
组织培养
无性系繁殖
繁殖技术
ild jujube,tissue culture ,phytohormone,clone porpagation
