摘要
经选育和诱变得到一株产苹果酸的黄曲霉菌TH5007,通过发酵条件的优化,以薯干粉的水解液为主要原料,发酵5dL-苹果酸可达到6%左右。在总酸中苹果酸含量平均达72%以上,杂有机酸主要是柠檬酸,延胡索酸的含量在0.02%以下。补糖发酵比分批发酵产酸性能更佳。
A strain of Aspergillus flauut TH5007 producing L-malic acid was isolatedand selected after a series of subsequent mutation. Various fermentation conditions werestudied to obtain process optimization, characterized by the direct fermentation with thehydrolysate from sweet potato powder. The concentration of L-malic acid in the fermentedbroth can reached to about 6.0%. Among the total acids in the ferinentation broth, Thecontent of malic acid amounts to above 72%. Citric aicd predominanted in the other organicacids than malic acid. The content of fumaric acid can be controlled below 0.02%. Produ-ction of L-malic acid with the fed-batch process is better than that with the batch process.
出处
《微生物学通报》
CAS
CSCD
北大核心
1995年第5期276-279,共4页
Microbiology China
关键词
L-苹果酸
发酵
黄曲霉菌
薯干粉
L-Malic acid, Direct fermentation process, Aspergillus flavus, Sweet