摘要
应用超薄层琼脂糖凝胶等电聚焦（ＵＬＡＧＩＦ），随机检测武汉地区５１１例健康成人红细胞酸性磷酸酶（ＥＡＰ）表现型，结果ＥＡＰ－Ａ型２４例（４．７０％），ＥＡＰ－ＢＡ型１７３例（３３．８６％），ＥＡＰ－Ｂ型３１４例（６１．４５％），计算出基因频率ＥＡＰａ＝０．２１６２，ＥＡＰｂ＝０．７８３８。经Ｈａｒｄｙ－Ｗｅｉｎｂｅｒｇ平衡公式检验Ｐ＞０．９９５，吻合度良好，频率分布与北京、西安、辽宁等地区对比差异无显著性意义，室温保存６周血痕ＥＡＰ分型无困难，为法医物证学提供了一项灵敏、可靠的检测方法。
A method of ULAGIF was used for EAP phenotype determination in 511 healthy,unrelated adults.Of the 511 individuals tested,24 EAP-A(4.70%)phenotypes,173 EAP-BA(33.86%)and 314 EAP-B(61.45%)phenotypes were observed.The calculated allelic frequencies of EAP in the unrelated samples are as follows:Eap￣a=0.216 2;Eap￣b=0.783 8.When the Hardy-Weinberg equilibrium test was performed,the observed and expected values were in good agreement(x￣2=0.007 5,P>0.05). The allelic frequency distribution in the Wuhan population did not differ significantly from those in Beijng,Liaoning and Xi'an.This study also showed that bloodstain kept at ambient temperature for 6 weeks could be typed without difficulty.A sensitive and reliable method for forensic serological examination has been presented in this paper.
出处
《同济医科大学学报》
CSCD
北大核心
1995年第4期269-272,共4页
Acta Universitatis Medicinae Tongji
关键词
超薄层
琼脂糖凝胶
等电聚焦
红细胞
酸性磷酸酶
isoelectric focusing
erythrocytes
acid phosphatase
gene frequency
phenotype
