摘要
用多聚酶链反应（ＰＣＲ）方法，采用一套针对第４基因的４种代表性等位基因的寡聚核苷酸引物对１６株人轮状病毒代表株进行了试验。结果证明对ＶＰ４基因型具有特异性。用此方法对１９９份日本和泰国的婴儿粪便样品进行了ＶＰ４的分型研究，其中Ｐ１Ａ型最多见，其次为Ｐ１Ｂ型；并分别在泰国和日本的样品中检出２株Ｐ２型和１株Ｐ３型。
representative cultivable human rotavirus strains were used to confirm the specificity of VP4 genotyping by polymerase chain reaction(PCR)using the nested oligonucleotides specific to each of the four representative gene 4 alleles.Using the PCR,we surveyed the gene 4 alleles of 199 human rotaviruses in stools collected from Japan and Thailand.It was found that strains with the gene 4 allele,corresponding to P1A serotype,were the most prevalent,and two strains with P2 gene 4 allele and one strain with P3 gene 4 allele were detected in samples from Thailand and Japan,respectively.
出处
《同济医科大学学报》
CSCD
北大核心
1995年第4期264-268,共5页
Acta Universitatis Medicinae Tongji
