摘要
家蚕Ｂｏｍｂｙｘｍｏｒｉ丝素蛋白经中性盐溴化锂处理后成为水溶性丝素，然后制成固定化过氧化物酶（ＰＯＤ）的丝素膜。经分光光度法分析表明这种固定化ＰＯＤ丝素膜的最适ｐＨ值范围较游离酶宽，为ｐＨ７．０～８．０；酶促反应温度在５０℃其活性最大。而且，此固定化酶膜的热稳定性良好，在６０℃保温１５分钟，其活性几乎未受影响，而游离酶失活４０％。因此，ＰＯＤ对ｐＨ和热的稳定性在丝素蛋白中固定后得到了改善，蚕丝丝素是一种良好的固定化酶的生物材料。
The aqueous solution of the silk fibroin from silkworm Bombyx mori cocoons was obtained by treatment of the neutral salt(LiBr). Peroxidase (POD) was immobilized in the silk fibroin membrane by immersion in 80% methanol aqueous solution.Optimum pH range of the activity of the POD-immobilized fibroin membrane.being at pH7. 0-8. 0,was widened in comparison with the case of free enzyme. The maximal activity for the immobilized enzyme was at reaction temperature 50℃ (pH7. 0),and its thermal stability was good since its activity was scarcely lost althrough the free POD lost about 40% of the original activity,when being incubated at 60℃ for 15min. Thus the stabilities of the enzyme VS. pH and heat were much improved by the immobilization in silk fibroin. Silk fobroin is a good biomaterial for immobilized enzyme.
出处
《苏州丝绸工学院学报》
1995年第2期99-103,共5页
Journal of Suzhou Institute of Silk Textile Technology
关键词
过氧化物酶
丝素膜
固定化
活性
桑蚕丝
fibroin,lmmobilized enzyme,POD-immobilized fibroin membrane
