摘要
介绍用高效液相色谱法分离测定鱼鱼油及其制品中的二十碳五烯酸（ｅｉｃｏｓａｐｅｎｔａｅｎｏｉｃａｃｉｄ，ＥＰＡ）和二十二碳六烯酸（ｄｏｃｏｓａｈｅｘａｅｎｏｉｃａｃｉｄ，ＤＨＡ）方法。使用Ｗａｔｅｒｓ液相色谱仪，μ－ＢｏｎｄｐａｒｋＣ＿（１８）Ｈ＿（３７）填充柱（３０ｃｍ×４ｍｍ）。鱼油经０．５ｍｏｌ／ＬＫＯＨ乙醇溶液皂化，三乙胺－ω－溴苯乙酮酯化后直接进样。以８８％的甲醇水溶液为流动相，紫外２５４ｎｍ检测，用外标法测定。该方法的回收率ＥＰＡ为９３．０％～９８．０％，ＤＨＡ为９３．０％～１００．０％。相对标准偏差ＥＰＡ为０．４１％～０．８６％，ＤＨＡ为０．６７％～１．２０％（ｎ＝７）。本法简便、快速、重现性好。
The method of high performance liquid chromatography analysis for eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in the anchovy oil was reported. The sample was determined with HPLC system of model Waters and μ-Bondpark C_(18) colume (30 cm ×4 mm i. d). The EPA and DHA in fish oil was saponified with 0. 5 mol/L KOH-C_2H_5OH,esterified with triethylamine-ω-Bromoacetopnenone, and inject directly into the system of HPLC. UV detection was performed at 254 nm,with the mobile phase of Methanol-water(88 : 12). The contents of the EPA and DHA in the sample were determined by standard curve. The recovery rates of EPA and DHA were 93. 0%～98. 0% and 93. 0%～100. 0% respectively.The relative standard deviations of EPA and DHA were 0. 41%～0. 86% and 0. 67%～1. 20% (n= 7). This method is simpie,rapid and with good repeatability.
