摘要
作者等采用改进的荧光光源及普通显微镜,于1994年7—8月间分别在四川省筠连县和名山县间日疟流行区,对吖啶橙染色技术检测疟原虫进行了现场应用研究。共检测210例门诊就医发热病人,与经典的姬姆萨染色检测结果比较分析,吖啶橙染色法的敏感性为97.2%,特异性为91.2%,两种方法的符合率为94.3%。检测速度可以提高2倍以上,明显降低了基层医院检验人员的工作强度。此种荧光光源结构简单,效能稳定,易于操作,价格较便宜,利于在基层医疗及防疫部门推广使用。
Acridine orange (AO) stain using an improved fluorescent light source, interference filter supported by Fumihiko Kawamoto of Japan and conventional light microscope were applied for diagnosing vivax malaria in endemic areas of Junlian and Mingshan counties during July and August, 1994. 210 cases of clinic patients with fever were examined and the results were compared with the Giemsa technique (GS). The sensitivity, specificity and concordance rates of AO stain technique were 97. 2%. 91.2%, and 94.3% respectively. The authors suggested that this kind of fluorescent light source would be practical to be used in local hospitals and health stations because of its simplicity, stability, low price and easy manipulations.
出处
《实用寄生虫病杂志》
CAS
1995年第3期117-119,共3页
Journal of Practical Parasitic Diseases
基金
UNDP/WorldBank/WHO/TDR提供基金
关键词
疟疾
吖啶橙染色法
间日疟原虫
病原学诊断
Plasmodium vivax, acridine orange, stain technique, field application