摘要
采用马来酰亚胺标记完整的大脑皮层细胞，观察由低氧引起的ＥＳＲ谱线的变化及脑细胞脂质过氧化程度，低氧引起细胞过氧化物生成增加，膜蛋白构象改变．金属硫蛋白（１０￣（－５）ｍｏｌ／Ｌ）能明显抑制过氧化反应，具有一定的抗氧化作用．并对实验的分子机理进行了简要的讨论．
Maleimide was used as spin labelfor studying the effect of hypoxia on rat cere-bral cortex neurons.Results showed that hy-poxia induced the lipid peroxidation in cerebralcortex cells and increased the formation ofLPO.By comparing the characteristics of ESRspectra,it was found that values of τ_c weresignificantly higher in hypoxic group than thatin control(P<0. 01).The increase in the ra-tio of*h_o/h_(-1) was well correlated with the en-hance in hypoxia group. It was also found thatMT could inhibit the lipid peroxidation incerebral cortex cells.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1995年第4期337-340,共4页
Progress In Biochemistry and Biophysics
基金
国家"八五"攻关课题
关键词
大脑皮层
神经元
膜蛋白
构象
电子自旋共振
cerebral cortex,neuron,elec-tron spin resonance techniques,spin label,hypoxia,metallothionein
