摘要
利用鼠鼠杂交瘤2F7细胞(分泌IgG2a单抗)研究了Pluronic F-68、甲基纤维素、羧甲基纤维素及聚醚多元醇与杂交瘤细胞生物相容性及添加限制浓度;研究了添加浓度对葡萄糖的利用及氨的生成影响;在高速搅拌、高剪切力下考查添加剂的保护效果。结果表明,0.05—0.10％(W/V)Pluronic F-68、0.10—0.20％(W/V)甲基纤维素能较好地保护杂交瘤细胞;高浓度Pluronic F-68增加了葡萄糖的比消耗速率及氨的比生成速率;高浓度甲基纤维素增加了氨比生成速率;羧甲基纤维素添加浓度低于0.1％不影响细胞生长,也无保护作用,羧甲基纤维素不影响细胞对葡萄糖的比消耗速率,但增加了氨比生成速率;聚醚多元醇分解细胞。在1.5升GelliGen生物反应器中,培养基添加0.10％Pluronic F-68、搅拌转速70r/min下细胞正常生长。
The potential toxicity and optimal concentrations of different protective agents such as pluronic F-68,methylcellulose (MC. ),CMC,and GPE on the growth in vitro of murine hybridoma 2F7 cell which secretes monoclonal antibody against small cell lung cancer were studied. The effects of adding protective agents on glucose utilization rate and ammonia production rate were investigated. The protective effects of different protective agent concentrations at high agitation speed were also observed. It is shown that 0. 05-0. 10%(W/V) pluronic F-68,0. 10-0. 20%(W/V) MC can protect hybridoma cell from shear stress at high agation speed. Adding pluronic F-68 can increase glucose utilization rate and ammonia production rate, while adding MC does not effect glucose utilization rate, but increase ammonia production rate. Although CMC does not effects 2F7 cell growth at the concentration less 0. 10%(W/V),but it exhibits no protective property. GPE can lyze hybridoma cell.. In 1. 5 L Celligen bioreactor, when pluronic F-68 concentration is 0. 10%(W/V) in medium and agation speed is 70r/min the hybridoma cell can grow normally.
出处
《生物工程学报》
CAS
CSCD
北大核心
1995年第2期120-125,共6页
Chinese Journal of Biotechnology
基金
国家"八五"攻关资助课题
