摘要
利用放射免疫测定法，观测了自然冬眠阵过程的常温、入眠、深眠、激醒四个阶段中，达乌尔黄鼠不同脑区及血浆中β－内啡肽（β－ＥＰ）与甲啡肽（ＭＥＫ）的含量。结果表明：黄鼠下丘脑、垂体中的β－内啡肽在入眠过程中增多，在深眠状态下的含量又高于入眠期；海马、血浆中的的β－内啡肽含量变动趋势正好相反，在入眠期与深眠期低于常温水平；桥延脑中含量在冬眠阵过程中变化不大。甲啡肽含量在入眠期升高，而在深眠期重新下降，不同脑区趋势相同但程度各异，以海马中的变动最为明显。提示不同类型的阿片肽在冬眠过程中的作用不同；不同脑区在冬眠调控中的参与程度各异。
Measurements of β-endorphin (β-EP) and Met-enkephalin (MEK) contents in var ious brain areas and plasma of ground squrriels were carried out by radioimmumoassay (RIA) through euthermia, entrance,deep hibernation and arousal phases of natural hibernation bout. The main results showed that,the β-EP content in hypothalamus and pituitary during the entrance phase were increased and further deepened during deep hibernation,whereas in hippocampus and plasma the tendency of the changes of β-EPcontent took place in an opposite direction,i.e.being lowered down from the euthermia level upon entering the entrance and deep hibernation phase. The content in pens and medulla showed little change through the bout.The contents of MEK were increased during entrance and lowered when enteringinto deep hibernation to different extent in different brain areas, being most significant change has taken place in hippocampus.These results indicated that the role of β-EP and MEK in hibernation was different both in regard to brain regional changes of their content and in the degree of thechanges.
出处
《生理学报》
CAS
CSCD
北大核心
1995年第6期610-614,共5页
Acta Physiologica Sinica
基金
国家自然科学基金
关键词
冬眠
冬眠阵
脑
血浆
阿片肽
β-endophin
met-enkephalin
hibernation bout
ground squrriels
RIA
