摘要
本文利用选择性肾动脉注射的方法和离体肾灌注(isohtedperfusedratkidney,IPK)技术,观察了内皮细胞衍生松弛因子(EDRF)的抑制剂N-nitro-L-irginine(L-NA),抑制肾组织局部产生EDRF后对正常大鼠肾功能的影响。结果表明,(1)选择性肾动脉注射L-NA后,大鼠血压无显著变化.肾血流量仅呈下降趋势,但尿量和尿钠排泄量明显减少(4.O±0.7vs5.1±0.8μmol/min,P<0.05);乙酰胆碱只能部分逆转其作用;L-NA则能减少乙酰胆碱引起的肾血流量增加。(2)L-NA能够直接降低离体肾灌注的尿量和尿钠的排泄。此外,高浓度的L-NA还可增加IPK的灌注阻力。上述结果有利于解释肾内EDRF在调节肾功能中的作用。
he purpose of this investigation was to testlocal renal effects of nitric
oxide inhibition with NG-nitro-L- arginine(L-NA) in normotensive rats.Intrarenal infusion of L- NA
(10 nmol/100 g BW/min) tended to lessen renal blood flow (10%) ,without changing RFG and
blood pressure. Mean-while, there was reduction in urine flow( UV) andurinary sodium excretion
(U_(Na) V) compared withcontralateral kidney. The renal vasodilatory and ex-cretion caused by
intrarenal inJection of acetycholinewere attenuated during L- NA infusion. By
isolatedperfusated rat kidney , at 100 nmol/L of L-NA bothUV and U_(Na)V were decreased as
compared with thecontrol period. The other hemodynamic parameterswere not altered with this
concentration. At 1000nmol/L of L-NA, RVR was increased by about20%, perfusion flow rate was
reduced by about10% ,moreover , UV and U_(Na)V were decreased sig-nificantly. These data
indicate that EDRF not onlyplays an important role in regulation of renal hemodynamics , but
can affect sodium and water handlingbv the renal tubules.
出处
《肾脏病与透析肾移植杂志》
CAS
CSCD
1995年第3期205-211,共7页
Chinese Journal of Nephrology,Dialysis & Transplantation