摘要
为了提高肿瘤浸润淋巴细胞治疗卵巢癌的临床效果，本实验分析研究了卵巢癌ＴＩＬ细胞存活天数与细胞增殖能力、细胞表型及杀伤力之间的关系，发现ＴＩＬ细胞第１５天出现增殖，至２５天达到高峰，４０天左右开始增殖缓慢；培养２０天的ＴＩＬ细胞表型中ＣＤ＿３、ＣＤ＿４、ＤＲ明显高于１０天的ＴＩＬ细胞，其中ＴＩＬ的ＣＤ＿３为８６．２％，ＣＤ＿４、ＤＲ也较高，分别为５５．６％，９３．１％，ＴＩＬ培养至３０天，其细胞表型变化幅度较小；培养至２０天的ＴＩＬ细胞已达较高的杀伤活性。提示培养２０～４０天的ＴＩＬ细胞比较适合临床应用。
In order to enhance the therapeutical effect, the authors studied on the relationshipamong survival, proliferation, phenotypes and cytotoxicity of TILs from ovarian can- cer and found out that TILs begin to proliferate at 15 day, reached to maximum at25 day and decreased slowly after 40 day. Results showed that CD_3(8.2%),CD_4(55.6%)and DR (93.1%)of TILs cultured at 20 day were higher than that of 10day. The change of phenotypes of TILs cultured from 20 day to 30 day was insigni-ficant. The cytotoxicity of TILs cultured at 20 day was strong. These suggest thatTILs cultured 20～40 day were suitable for clinical treatment.
出处
《上海医学》
CAS
CSCD
北大核心
1995年第6期325-327,共3页
Shanghai Medical Journal
