摘要
苗期人工接种，对转化ＣＭＶ－ｃＰ基因的番茄品种８８０５Ｒ１～Ｒ４代进行接种ＣＭＶ病毒试验。结果表明：黄瓜花叶病毒外壳蛋白基因在番茄中的表达，对抑制ＣＭＶ病毒病症状的表现及延迟其发展均起到一定作用。８８０５Ｒ３代对ＣＭＶ的抗性明显高于Ｒ１代，Ｒ４代抗性已基本稳定。当高浓度的ＣＭＶ病毒侵染Ｒ４代植株时，也会出现１００％的发病率，但它们的新生枝叶能正常生长并开花结果。利用半叶法测定８８０５Ｒ。（ｃｐ＋）及８８０５Ｒ４（ｃｐ－）接种叶和新生叶病毒浓度，结果是：ｃｐ（＋）和ｃＰ（－）在接种叶上病毒含量相当，但ｃＰ（＋）的新生叶中病毒含量明显低于ｃＰ（＋）的等位叶，说明ＣＭＶ－ｃＰ基因的存在，限制了ＣＭＶ病毒粒子的迁移和繁衍，最终也减轻了ＣＭＶ病毒的危害程度。
The transgenic plants of tomato 8805R1￣R4 progenies were inoculated with CMV virus at seedling stage. It was shown that the expression of the CMV-cp gene in the transgenic plants resulted in the protection of these plants from infection by CMV. The systemic infection of the virus was reduced or delayed,compared with the control plants. The resistance of 8805R3 progeny was significantly better than that of 8805R1 and the identical resistance to CMV was obtained in 8805R4.The 100% infected plants appeared in 8805R. progeny,when the seedlings were inoculated with CMV virus at higher concentration,but their new shoots and leaves grew vigorously and fruit-setting was normal.The CMV virus contents in the inoculated leaves and systemic leaves of 8805cp(+)and 8805cp (-)were tested by half-leaf method. It was concluded that the CMV content in thenewborn leaves of 8805cp(+) was remarkably lower than that of 8805cp (-).
出处
《上海农业学报》
CSCD
1995年第1期73-77,共5页
Acta Agriculturae Shanghai
