摘要
担子菌ＬＭＰＱ３９ Ｐｓｔ Ⅰ片段基因库的构建王灿华，吴其威，黄瑞珊，朱章玉，李堃宝（生物技术研究所）关键词担子菌ＬＭＰＱ３９，ＰｓｔⅠ酶切，质粒ｐＢＲ３２２，克隆库中图法分类号Ｑ７５当今的育种工作，除使用常规手段外，主要是从分子水平和细胞水平两个层...
Basidiomycetes LMPQ39 total DNA was digested with Pst I and 4～5kb DNA fragments obtained by low melting point agarose gel electrophoresis. The clon bank of LMPQ39 total DNA Pst I fragments has been constructed using PBR 322 DNA as the clonning vector and E·coli TG I as the recipient. The number of recombinants was 2. 52× 105. 14 of those clons were chosen randomly and digested with Pst I. 9 clons were found to be different from the vector PBR 322. At the same time, the necessary number of recombinants has exceeded the volume needed theoretically that represents the clon bank of LMPQ39.
出处
《上海交通大学学报》
EI
CAS
CSCD
北大核心
1995年第2期161-164,共4页
Journal of Shanghai Jiaotong University
基金
国家八.五攻关课题
