摘要
近几年来发现,中性粒细胞在多种疾病的发病学中有广泛的作用。粒细胞弹性蛋白酶(neutrophil elastase, NE)是粒细胞嗜天青颗粒中的一种作用范围广、水解能力强的蛋白水解酶,与急慢性炎症及多种组织损伤性疾病的发生有密切关系。我们建立了一种测量人血浆NE的酶免疫——生物素法,需血浆量为20μl。正常不吸烟成人血浆NE含量为33.7±6.7ng/ml((?)±SD);慢性阻塞性肺疾患病人为48.0±10.9ng/ml;皮肤化脓性感染病人为69.1±15.7ng/ml,系统性红斑狼疮病人为61.0±13.9ng/ml;而再生障碍性贫血病人仅12.3±5.3ng/ml,这些结果与正常人相比均有显著差异。作者认为;本方法特异性好,灵敏度可达毫微克水平,可望作为探讨NE在疾病发生中的作用及监测某些疾病变化的较好指标。
The role of neutrophil in the pathogenesis of many diseases attracts more attentions than before. Neutrophil elastase (NE), a powerful neutrol proteinase capable of causing major tissue destruction in many diseases, is mainly present in the azurophil granules of neutrophil. A solid-phase enzyme-linked immunosorbent-biotin assay for human NE has been developed. Only 20μl of plasma sample is needed. Non-smoking healthy adults have 33.7±6.7ng NE/ml plasma; patients with chronic obstructive pulmonary diseases, 48.0+ 10.9 ng NE/ml; patients with pyogenic dermatisis, 69.1±15.7 ng NE/ml; patients with systemic lupus erythematosus, 61.0±13.9 ng NE/ml; patients with aplastic anemia, only 12.3±5.3 ng NE/ml. All of these results have significant differences compared with the result of healthy adults. The results of assay showed that this quantitative assay with good specificity and accuracy may be a better criterion for investigating the effect of NE on pathogenesis of some diseases.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1989年第4期193-198,共6页
Chinese Journal of Pathophysiology
关键词
粒细胞
弹性蛋白酶
酶免疫测定法
Human neutrophil elastase
Enzyme-linked immunosorbent assay(ELISA)
Avidin-biotin-horseradish peroxidase complex