摘要
以小叶杨（Ｐｏｐｕｌｕｓｓｉｍｏｎｉｉｃａｒｒ．）原生质体培养的愈伤组织为供试材料，用高压波相色谱法（ＨＰＬＣ），测定它们在四种不同分化培养基上四大类内源激素（ＩＡＡ，ＧＡ３，ＣＴＫ，ＡＢＡ）的含量变化，并检定有关Ｎ的生化反应。结果表明，不同分化培养基上的愈伤组织中内源激素含量和种类差异很大。导致细胞分裂素（ＣＴＫ）含量高，种类多以及细胞分裂素与生长素的比值（ＣＴＫ／ＩＡＡ）高的处理，有利于愈伤组织分化；但若愈伤组织中含有内源脱落酸（ＡＢＡ），则抑制分化。测定结果还表明，小叶杨原生质体培养的愈伤组织分化过程中，其硝酸还原酶活力与氨基态Ｎ含量互为消长。分化频率高的愈伤组织中，硝酸还原酶活力低，而氨基态Ｎ含量高．
In the protoplast culture of Popelys simonii Carr.,the variations in con tents of IAA,GA3,CTK,ABA four kinds of endogenous hormones in the calli differentiated from four differentiation culture media were determined by the high pressure liquid chromatography method,and the biochemical reactions of nitrogen related to them were also tested.It was shown that there was a great change in the contents and kinds of the endogenous houm ones among the calli differentiated from the different media.The treatment which resulted in the higher content and more kinds of CTK, and the higher ratio of CTK/IAA within the calli was favourable to the calli different iation, whereas if the ABA was contented in calli,the differentiation of the calli was inhibited. During their differentiation process,a mutually exclusive relation between the activity of nitrate reductase(NR) and the amino-N content was found, in the calli with higher differentiation frequancy the NR activity was low,but the amino-N content was high.
出处
《林业科学》
CAS
CSCD
北大核心
1995年第1期1-7,共7页
Scientia Silvae Sinicae
关键词
小叶杨
原生质体培养
内源激素
分化频率
Populus simonii carr.,Protoplast culture,Endogenous hormones, Differentistion frequency
