摘要
利用间接免疫荧光的细胞化学技术对番茄、黄瓜、菠菜、甜菜及小麦等不同抗寒性植物微管的冷稳性进行了比较研究。结果指出,不抗寒的喜温性植物番茄和黄瓜的气孔保卫细胞的微管在0℃—1℃冷处理3小时即解聚;属于中等抗寒性植物的菠菜和甜菜幼苗经秋季低温锻炼后,其气孔保卫细胞的微管在0℃和—5℃低温处理3小时,均不发生解聚;具有较强抗寒性的冬小麦品种农大139幼苗在2—3℃低温锻炼期间,微管结构保持完整,经过15天低温锻炼的幼苗在-8℃冰冻处理3小时,微管也不受破坏。这些结果表明,微管的冷稳性与植物的抗寒性成正相关。
A comparative study on the microtubule cold stability of tomato (Lycopersicum esculen- tum),cucumber (Cucumis sativus),spinach (Spinacia oleracea),beet (Beta saccharifera) and wheat (Triticum aestivum) was carried out by immunofluorescent microscopy.The results indicated that the depolymerization of microtubules in the stomatal guard-cells of chilling sen- sitive plants,tomato and cucumber occurred under the chilling treatment of 0℃—1℃ for 3 hrs, but,in mediate cold rtsistant plantS,spinach and beer did not so when their cold acclimated se- edlings were treated for 3 hrs at 0℃ and-5℃.The microtubules in the stronger cold resis- tant winter wheat variety Nongta 139 were maintained in low temperature acclimating period, and could not be destroyed after the cold acclimated seedlings were frozen at -8℃ for 3 hrs. The results suggest that the microtubule Cold stability is closely related to the cold hardiness of plants.
基金
国家自然科学基金资助的课题
关键词
微管
植物抗寒性
免疫细胞化学
Microtubule
Microtubule cold stability
Plant cold hardiness
Immunocytochemistry
