摘要
用三种酶液游离大量高活力的紫花苜蓿实生苗根原生质体，Ｋａｏ的原生质体培养基使根原生质体分裂形成细胞团。原生质体形成的愈伤组织在ＵＭ培养基上分化体细胞胚。体细胞胚在无激素的ＭＳ琼脂培养基上发育为完整植株。
Protoplasts were released from roots of seedling of Medicago sativa by three enzyme solutions.Root pro-toplasts of Medicago sativa divded to form colonies(small calli)in Kao' s protoplast cultuer medium. Proto-plast-derived calli developed somatic embryos on UM medium. Somatic embryos developed into whole plantson hormone free MS medium.
出处
《科技通报》
1995年第4期232-234,共3页
Bulletin of Science and Technology
