摘要
目的:探讨白介素-1β(IL-1β)、白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)对癫痫的神经免疫调节作用及卡马西平(CBZ)对它的影响。方法:180只Wistar大鼠随机分为对照组、红藻氨酸(KA)组和CBZ组,后两组再按癫痫发作后的不同时点分为6个亚组(1h、4h、12h、24h、48h和72h);以KA建立癫痫模型,采用放射免疫分析(RIA)测定IL-1β、IL-6和TNF-α的水平变化。结果:癫痫发作后72h内,血清和脑匀浆液中IL-1β、IL-6和TNF-α的水平变化不同;在不同时点,KA和CBZ组的三者水平均明显高于对照组(P<0.01或P<0.05),同时,CBZ组的三者水平明显低于KA组(P<0.01)。结论:癫痫大鼠免疫系统处于活化状态,细胞因子水平的失衡可能参与了癫痫的免疫病理过程;CBZ对癫痫具有一定的免疫抑制作用。
Objective To investigate the neuro - immuno - modulation effect of the eytokines IL-1β, IL - 6 and TNF-α as well as the influence of carbamazepine （CBZ） in experimental rat models with epilepsy. Methods Sixty rat models of epilepsy （KA group） were prepared with intraperitoneal injection of kainic acid （10mg/kg）. Another 60 models （CBZ group） were prepared with the same injection but pre - treated with CBZ garage （45mg/kg） daily for 7 days. Sixty rats with sham injection and sham garage were used as controls. In the two model groups, ten animals were sacrificed at each of the time points ： 1,4, 12, 24, 48, 72h after the development of epilepsy and the serum and brain homogenate contents of the cytokines were repeatedly determined with RIA. The same was applied to the control group at the corresponding time point. Results In the two epileptic models, serum and brain homogenate contents of these cytokines increased gradually and attained peak levels at 24h （TNF-α） or 48h （IL -1β, IL -6）. The levels in the KA group at any time point were significantly higher than those in the CBZ group and control group （P 〈0.01 ）. Levels in the CBZ group were also significantly higher than those in controls （P 〈 0.05, P 〈0.01 ）. Conclusion The immune system was greatly activated in the rat epileptic models, carbamazepin might provide some protective action through immunosuppression.
出处
《放射免疫学杂志》
CAS
2005年第4期256-259,共4页
Journal of Radioimmanology
