摘要
根据GenBank中收录的鸡白细胞介素-17(ChIL-17)基因的cDNA序列设计了1对特异性引物,以经ConA刺激3h的鸡脾淋巴细胞总RNA为模板,用RT-PCR方法成功克隆了ChIL-17的cDNA.该cDNA全长725bp,其中第2～508位是该基因的阅读框(ORF),共编码169个氨基酸.与已报道的序列相比较,二者核苷酸序列的同源性为99.6%(722/725),共有3个碱基发生变异,其中有2个变异位于阅读框之内.DNAssit软件分析表明,二者推导的氨基酸序列同源性为100%.同时,以鸡脾淋巴细胞DNA为模板,用PCR方法首次扩增获得了ChIL-17的基因组DNA序列,经序列分析,其序列全长1934bp,由2个内含子和3个外显子组成,3个外显子分别位于第2～27、569～815和1484～1720bp处.
The cDNA sequence encoding chicken interleukin-17 （ChIL-17） was cloned from the spleenic lymphocytes of Huiyang chicken by means of using the specific primers based on the reported sequence in GenBank. The full length of the cloned cDNA was 725 bp with an ORF ranging from 2nd to 508th bp, which encodes 169 amino acids. The sequence of the ChIL-17 gene had 3 bases variation aligned with the GenBank sequence and with an identity of 99. 69% （722/725）. The genomic DNA of this ChIL-17 gene was cloned using the same pair of primers, and is 1934 bp in its full length with 2 introns and 3 exons.
出处
《中国兽医科技》
CSCD
北大核心
2005年第8期643-647,共5页
Chinese Journal of Veterinary Science and Technology
基金
国家高技术研究发展计划(863)项目(2002AA241331)
广东省农业科学院博士启动基金项目
