摘要
目的研究内皮细胞表达的CD137分子与活化的T细胞表达的CD137L交联后对T细胞的刺激效应。方法将活化的内皮细胞与活化的T细胞共培养,ELISA检测共培养上清中IL-2、IL-10和IFN-γ的含量;流式细胞仪检测内皮细胞表面标志分子CD25、CD69的表达变化;CCK-8试剂盒检测T细胞存活率,并观察用融合蛋白抗-CD137阻断CD137-CD137L共刺激通路后T细胞分泌细胞因子的变化。结果采用细胞共培养后,T细胞分泌IL-2、IL-10和IFN-γ的水平下降,而且CD25、CD69的表达水平降低,T细胞存活率下降,应用抗-CD137单克隆抗体阻断CD137-CD137L相互作用后,IL-2、IL-10和IFN-γ的分泌水平升高,CD25、CD69的表达上调,T细胞存活率上升。结论活化的内皮细胞诱导表达CD137蛋白分子与活化的T细胞上诱导表达的CD137L相互作用,通过CD137L向T细胞内传递抑制信号,抑制了T细胞合成和分泌细胞因子的功能。表明CD137-CD137L信号通路在内皮细胞与T细胞的信号作用中有着重要的地位。
Objective To detect that CD137 expressed by human umbilical vein endothelial cells （HUVECs） provides costimulatory signals to inhibit the function of synthesizing cytokines and the release of activated T cells by its combination with CD137L from activated T cells. Methods HUVECs were cultured with activated T cells, and the level of IL-2, IL-10 and IFN-γ in the supernanant of the cocuhure and the expression of CD25, CD69 on activated T cells and the viability of T cells were examined. Results The secretion of IL-2, IL-10, IFN-γ were obviously decreased by activated T cells. The expression of CD25, CD69 was down-regulated on activated T cells, and the viability of T cells also decreased. However, the blocking of CD137-CD137L interaction by anti-CD137 monoclonal antibodies enhanced the expression and the release of these cytokines. Conclusion CD137L on activated T cells mediates an inhibitory signal to T cells and leads to decrease the synthesis and secretary of cytokines. CD137-CD137L plays a critical role in the interaction of HUVECs and T cells.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2005年第15期1541-1543,共3页
Journal of Third Military Medical University
基金
国家重点基础研究发展规划资助项目("973项目"
G1999054203)
国家自然科学基金资助项目(30271246)~~
