摘要
为了探讨乙酰胆碱受体（ＡＣｈＲ）抗体阳性重症肌无力患者血浆中的抗体滴度与临床症状之间不平行的原因，作者选用１０例抗体阳性患者作为研究对象，利用ＰｒｏｔｅｉｎＧ层析柱选择性吸附ＩｇＧ的原理从患者血浆中分离ＩｇＧ和非ＩｇＧ两部分，分别观察此两部分对ＴＥ６７１细胞表达的乙酰胆碱受体功能的影响。结果表明，ＩｇＧ部分对ａ－ＢｕＴｘ结合试验具有明显的抑制作用，ＣＰＭ值仅为１５００±２５０（ｎ＝５），非ＩｇＧ部分的抑制作用甚微；ｌｇＧ和非ＩｇＧ部分，两者均对ＡＣｈＲ功能有抑制作用，分别为５８±５％（ｎ＝５）和２５±５％（ｎ＝５）。研究结果提示，非ＩｇＧ部分对ＡＣｈＲ的抑制作用可能是抗体阳性患者抗体滴度与临床症状不平行的原因之一。因此作者认为进一步探讨非ＩｇＧ部分对ＡＣｈＲ的作用机制有助于揭示抗体阳性患者的发病机制。
ln order to study why seropositive myasthenic patient's clinical symptoms do not correlatewith anti-acetylcholine receptor(AChR)antibody titre, 10 cases of myasthenia gravis were selected as thestudy subect to observe the effect of lgG and non-IgG fractions purified from their plasma on the functionof AChR.ResuIts showed that a-BuTx bound to AChR was significantly suppressed by lgG fractions , butit was not inhibited by non-IgG。 BOth lgG and non-IgG fractions were found to have inhibiting effect oncarbachol induced sodium influx with their percentage being 58±5%(n=5)and 25±5%(n=5)respec-tively,It suggests that the inhibiting effect of non-IgG fractions on AChR function may be one of the reasons why seropositive myasthenia gravis clinical symptoms do not correlate all well with AChR antibodytitre。 Therefore,the effect of non-IgG fractions on AChR function may be helpful for the further study ofthe pathOgenesis of myasthenia gravis。
出处
《解放军医学杂志》
CAS
CSCD
北大核心
1995年第3期174-177,共4页
Medical Journal of Chinese People's Liberation Army
