摘要
目的通过筛选cDNA文库识别华支睾吸虫新基因,为进一步研究其功能及其应用奠定基础。方法对华支睾吸虫cDNA文库进行筛选,通过Blastn程序进行序列比对,识别华支睾吸虫新基因,并应用软件程序对其进行分析。结果筛选到一个475bp的核酸序列,编码153aa,理论分子量为16.9kDa,理论等电点(pI)为6.19,疏水氨基酸(AILFW V)占36%,带电荷氨基酸(RKH YCDE)占30%,极性氨基酸(NCQSTY)占28%,酸性氨基酸(DE)和碱性氨基酸(KR)各占9%。经BLAST分析,该蛋白属于M L域(M D-2-related lipid-recognition)家族,是一个新的分泌蛋白基因。结论发现华支睾吸虫分泌蛋白基因,可能有作为诊断候选抗原的价值。
Objective To identify and characterize the new genes of Clonorchis sinensis from a cDNA library.Methods The eDNA library of Clonorchis sinensis was constructed and screened. The cDNA sequences were analyzed with the tblastx and blastn programs on the NCBI website. Results A novel eDNA sequence encoding a secretory protein was identified. Translation of the nucleotide sequence revealed a putative open reading frame of 475bp with a molecular mass of 16.9kDa, pI 6.19 and 36% of “AILFWV”, 30% of “RKHYCDE”, 28% of “NCQSTY”, 9% of each “KR” and “DE”. As a result the protein is a putative member of the MD-2-related lipid-recognition family. Conclusion We have identified a novel gene of Clonorchis sinensis encoding a secretory protein, which is a putative member of the MD-2-related lipid-recognition family.
出处
《热带医学杂志》
CAS
2005年第5期574-575,共2页
Journal of Tropical Medicine
基金
广东省自然科学基金(重点)项目(No:36627)
广州市东山区科技攻关项目(No:2004-GX-18)
广东省科技攻关项目(No:2004B36001027)。
关键词
华支睾吸虫
分泌蛋白
克隆
Clonorchis sinensis
secretory protein
clone
