摘要
背景:创伤修复期间胃肠道存在再灌注损伤和灌注不全,引起肠黏膜损伤。目的:观察人用等效剂量参附注射液对创伤修复期间家兔肠pH、肠黏膜一氧化氮、丙二醛、Ca2+含量、血清双胺氧化酶的影响。设计:以实验动物为观察对象的随机对照实验。单位:武汉大学人民医院麻醉科。材料:实验于2003-08/10在武汉大学人民医院麻醉学研究室完成,选择健康家兔24只随机分为3组:参附注射液治疗组、单纯创伤修复组和对照组,每组8只。干预措施:通过股动脉放血犤2mL/(kg·min)犦,平均动脉压降至40mmHg并持续60min,回输自体血及等量平衡盐液制备兔创伤修复模型;参附注射液组于回输自体血同时先静注参附注射液2.1mL/kg,随后持续注入参附注射液5mL/(kg·h)。主要观察指标:分别于实验前,创伤修复1h,及再灌注1,3h检测乙状结肠黏膜内pH、肠黏膜一氧化氮、丙二醛及钙含量、血清双胺氧化酶活性。结果:24只家兔均进入结果分析。①参附注射液治疗组再灌注1,3h肠黏膜pH为7.171±0.102,7.194±0.106,高于单纯创伤修复组(6.920±0.155,6.971±0.165,P<0.05,P<0.01)及对照组(7.329±0.038,7.322±0.101,P<0.05)。②参附注射液治疗组再灌注1,3h血清双胺氧化酶为(35.090±1.184),(32.440±2.884)μkat/L,显著高于单纯创伤修复组犤(50.994±2.684),(52.377±1.217)μkat/L,P<0.01犦及对照组犤(15.970±1.734),(16.620±0.767)μkat/L,P<0.05犦。③再灌注3h肠黏膜一氧化氮、丙二醛含量参附注射液治疗组均显著低于单纯创伤修复组犤(61.8±5.3,72.2±5.8)μmol/g,(68.2±4.9,96.9±8.5)μmol/L,P<0.05犦。再灌注3h肠黏膜Ca2+含量参附注射液治疗组为(2.43±0.27)μmol/L,低于单纯创伤修复组犤(2.93±0.34)μmol/L,P<0.05犦,高于对照组犤(2.26±0.31)μmol/L,(P<0.05)犦。结论:给予家兔人用等效剂量的参附注射液,增加了肠黏膜灌注及氧合作用,抑制了肠黏膜一氧化氮活性和清除氧自由基及减轻钙�
BACKGROUND： Reperfusion injury and inadequate perfusion exist, causing the injury of intestinal mucosa during shock / resuscitation. OBJECTIVE： To investigate protective effect of shenfu injection （SFI） in equal dosage for human effect on intestinal mucosal pH （PHi） of sigmoid colon, the contents of nitric oxide （NO）, malonialdehyde（MDA） and Ca^2＋ of intestinal mucosa, activity of serum diamine oxidase（DAO of rabbits during shock/resuscitation. DESIGN：A randomized controlled trial with experimental animals as the subjects. SETTING： Depart ment ofAnesthesiology, Renmin HospitalofWuhan University MATERIALS： The experiment was performed in the Research Institute of Anesthesiology, Renmin Hospital of Wuhan University from August to October in 2003. Twenty-four adult healthy white rabbits were randomly divided into 3 groups with 8 animals for each： Shenfu injection group （SFI group）, shock/resuscitation group and control group . INTERVENTIONS： Hemorrhagic shock was induced by blood withdrawal from femoral artery at a rate of 2 mL/kg per minute until MAP dropped to 40 mmHg. MAP was maintained at this level for 60 minutes, then the collected auto-blood and the same volume of balanced salt solution was transfused to the animal for preparing shock/ resuscitation model. In SFI group： the animals were given 2.1 mL/kg SFI together with the auto-blood transfusion and followed by a continuous infusion of 5mL/kg SFI per hour. MAIN OUTCOME MEASURES： Intestinal mucosal pH （PHi） of sigmoid colon, NO, MDA and Ca^2＋ contents of intestinal mucosa, activity of serum DAO were detected before shock （So）, 1 hour after shock （S1）, 1 hour （R1） and 3 hour （R3） following resuscitation. RESULTS： Twenty-four rabbits entered the result analysis. ① PHi at the R1 reperfusion （7.171±0.102）,aml R3 reperfusion （7.194±0.106） of the SFI group were higher than those of the shock/ resuscitation group （6.920±0.155,6.971±0.165,P 〈 0.05,P 〈 0.01） and those of the contro
出处
《中国临床康复》
CSCD
北大核心
2005年第26期257-259,共3页
Chinese Journal of Clinical Rehabilitation
基金
湖北省教委科学基金资助(2003x125)~~
