摘要
目的研究乙肝病毒(HBV)慢性感染者外周血单个核细胞(PBMCs)特异性分泌γ-干扰素(INF-γ)的免疫反应。方法32例HBV慢性感染者,转氨酶升高的HBV免疫清除期患者及转氨酶正常的HBV免疫耐受期患者各半,常规方法分离PBMCs,体外用HBcAg或HBsAg刺激,并以1640培养基作为对照,用酶联免疫斑点法(ELISPOT)检测PBMCs分泌INF-γ的免疫反应。结果受HBcAg刺激87.5%(14/16例)免疫清除期的慢性HBV慢性感染者PBMCs的INF-γ分泌实验阳性,而所有免疫耐受期的HBV慢性感染者PB-MCs的INF-γ分泌实验阴性,二者之间差异有显著性(P=2.55×10-7);而受HBsAg刺激,二组患者之间差异没有显著性(P=0.11)。结论HBV免疫清除期与免疫耐受期患者PBMCs特异性分泌INF-γ的免疫反应不同。
Objective To investigate the interferon-γ（1FN-γ） production of peripheral blood mononueler cells （PBMCs） in response to hepatitis B core ant igen （HBcAg） and surface antigen （HBsAg） in chronic hepatitis B inffecfion. Metheds 32 patients chronically infected with HBV , 16 patients in clerance period （with the increased alanine aminortansferase ） ,were included . The PBMCs from the patients got by density gradient centrifugation were stimulated with HBcAg and HBsAg separately in vitro. The production of IFN-γ were measured by the method of en-zyme link immunal spot （ELISPOT）. Results In compare with the production of IFN-γ stimulated with 1640, those stinualated with HBcAg were significant higher for the 87.5% （ 14/16 cases）patients in clerance period and no change for all of the patients in tolerance period ,there was significant difference between them. Stimulation with HBsAg ,there was no singificant difference between two group （ P = 0. 11）. Conclusion The response of the production of IFN-γ stimulated with HBcAg for the patients in clerance period is significant different from those in tolerance period.
出处
《临床消化病杂志》
2005年第4期161-163,共3页
Chinese Journal of Clinical Gastroenterology
