摘要
目的探讨异种心脏移植后急性血管性排斥反应期移植心心肌中供、受者的组织因子与凝血的关系。方法供者为清洁级豚鼠,受者为清洁级SD大鼠。随机配对平均分为6组。建立异种心脏移植急性血管性排斥反应期动物模型。分别于移植术后0(未行心脏移植)、4、8、12、16和24h切取移植心脏,使用免疫组织化学染色法观察移植心的心肌凝血程度;同时采用逆转录聚合酶链反应(RT-PCR)监测移植心心肌中组织因子的表达。结果移植后8h,移植心心肌凝血程度明显,并随移植时间延长逐渐加重。供者组织因子mRNA表达在移植后4h最强,随后逐渐减弱,至移植后16h消失;受者组织因子mRNA表达在移植后16h出现,随后逐渐增强。结论移植心心肌中的组织因子在异种心脏移植后急性血管性排斥反应期凝血中起重要作用,其中供者的组织因子可能与凝血的激发有关,受者的组织因子可能与凝血的加重有关。
Objective To investigate the role of tissue factor in the occurrence and development of coagulation in acute vascular rejection of heart xenotransplantation. Methods An animal model for acute vascular rejection of heart xenotransplantation was established by using rat as recipient and guinea pig as donor. The xenografts were removed at 4, 8, 12, 16, and 24 h after the operation for fibrin sedimentation by irnmunohistochemical technique to evaluate coagulation. Meanwhile, the expression of tissue factor mRNA was detected at different time points by RT-PCR. Hearts of normal guinea pigs were used as controls. Results Immunohistochemical examination showed that coagulation developed at the 8 h when interstitial fibrin deposition and fibrinous thrombus appeared and aggravated with time. High expression of guinea pig tissue factor mRNA appeared at 4 h after the transplantation and then steadily declined and totally terminated at the 16 h. The expression of rat tissue factor mRNA appeared 16 h post operation and then kept steadily enhanced. Conclusions The tissue factor acts as an important role in the acute vascular rejection of heart xenotransplantation. The high expression of donor tissue factor mRNA might be related to the trigger of coagulation and the high expression of the receptor＇s tissue factor may be related to the further development of coagulation.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2005年第8期474-476,共3页
Chinese Journal of Organ Transplantation
基金
上海市卫生局青年基金项目(131014Y9)
