摘要
目的比较致死样哮喘(NFA)缓解期患者与健康人中常见的呼吸道病毒的分布差异。方法研究对象包括NFA缓解期患者12例,轻症哮喘患者21例及无哮喘病史的健康人52名。诱导痰用于RNA及DNA的提取,PCR及RT-PCR方法用于7种常见的呼吸道病毒,微小病毒(HRV),流感病毒A、B,副流感病毒1、3,呼吸道合胞病毒及腺病毒的检测。结果NFA缓解期患者的病毒检出率为42%(5/12),而轻症患者为24%(5/21),健康人为10%(5/52)。NFA患者的病毒检出率显著高于健康人。微小病毒、流感病毒A、B可存在于NFA缓解期患者的下呼吸道内。而健康人呼吸道内以微小病毒为主。哮喘患者气道内存在2种以上病毒定植的情况。结论呼吸道病毒可定植于哮喘患者及健康人的气道内而不诱发呼吸道症状,呼吸道病毒定植可能与哮喘的严重度有关。
Objective To compare the prevalence and spectrum of common respiratory viruses among patients with near-fatal asthma （NFA）, asthma and healthy controls. Methods Twelve near fatal asthmatics, 21 asthmatics and 52 healthy controlswere recruited in our study. Induced sputum from the study subjects was obtained for DNA and RNA extraction, PCR and RTPCR based techniques were applied to detect the 7 common respiratory viruses, including picornavirus, influenza A, B, parain-fluenza Ⅰ,Ⅲ, respiratory synsicial virus and adenovirus, Results The prevalence of single virus was detected in 42% （5/I2）in near fatal asthma group, 24% （5/21） in asthma group and 10% （5/52） in healthy control group. Virus was more prevalent in NFA than that in controls. Picornavirus, influenza A, B was commonly identified in the asthmatics, while picornavirus dominated in the healthy controls. Conclusions Respiratory viruses could exist in respiratory tract in asthmatics and healthy individuals without causing any symptoms. Respiratory viruses may be associated with asthma severity.
出处
《中国抗感染化疗杂志》
2005年第4期242-244,共3页
Chinese Journal of Infection and Chemotherapy
关键词
呼吸道病毒
逆转录-聚合酶链反应
Respiratory virus
Reverse transcript polymerase chain reaction
