摘要
双向凝胶电泳分析技术已在生物科学各领域被广泛应用,蛋白质组作图的意义已经日益显现。通过对4种常见尸食性蝇类初孵幼虫蛋白质组双向凝胶电泳和图象分析,发现各种类间双向凝胶电泳图谱差异显著,并对相应的等电点和相对分子量进行聚类和判别分析,结果表明,建立合适的尸食性蝇类初孵幼虫的双向凝胶电泳图谱可用于鉴别形态学极易混淆的昆虫种类。
With the application of two-dimensional gel electrophoresis （2-DE） technology in biology science, the importance of constructing proteome map has been recognized. Protein maps of neonate larvae of four common carrionbreeding fly species were obtained using 2-DE in the range of pH 4 - 7. After silver stained detection of protein spots, we found that there were marked differences among these maps. Isoelectric points and molecular weight analysis indicated that 2-DE map could be used to identify neonate larvae of common carrion-breeding flies which are confused in morphology.
出处
《昆虫学报》
CAS
CSCD
北大核心
2005年第4期576-581,共6页
Acta Entomologica Sinica
基金
国家自然科学基金项目(39870681)
关键词
蛋白质组
双向凝胶电泳
法医昆虫学
尸食性蝇类
种类鉴别
Proteom
two-dimensional gel electrophoresis
forensic entomology
carrion-breeding flies
speciesidentification
