摘要
目的:探讨肺炎克雷伯杆菌反复感染在慢性阻塞性肺疾病(COPD)形成中的机制。方法:将大鼠随机分为对照组(C)和肺炎克雷伯杆菌感染组(K)。动态观察肺感染K组肺细胞损伤改变。以免疫组化和原位分子杂交方法分别检测肺内各细胞c-fos蛋白和mRNA表达的变化。结果:K组第8周少部分细支气管管壁有平滑肌细胞和纤维组织增生,第16周纤维组织明显增生穴P<0.01雪。K组感染1周后,在不同时间内肺内各细胞均有c-fos蛋白和mRNA表达增加(P<0.01)。并且各自c-fosmRNA转录水平和c-fos蛋白表达水平基本平行,其中巨噬细胞最早表达c-fos蛋白。从第1天持续到感染第16周。结论:肺炎克雷伯杆菌感染在体内能诱发肺内各细胞损伤和c-fos转录和表达上调,在加重COPD形成中有重要作用。
Objective： To study the lung c-los mRNA transcription and protein expression inuced by Klebsiella Pneumoniae infection in rats, and discuss its pathogenesis in chronic obstructive pulmonary disease （COPD）. Methods： Rats were randomly divided into two groups including control group （group C） and Klebsiella Pneumoniae infection group （group K）.Lung cell injury changes were observe dynamically in group K. c-los mRNA transcription and protein expression of lung varied cells were measured by immunohistochemistry and hybridization. Results： h few airway bronchiole smooth muscle ceils and fibrous tissue proliferated slightly 8 weeks ater infection, and fibrous tissue obviously proliferated 16 weeks after infection in group K （P 〈0.01）. c-fos protein an mRNA expression of different time significantly increased 1 week after infection （P 〈 0.01） and c-fos mRNA transcription and protein expression levels were basically parallel, c-fos protein was first expressed by Phagocyte from 1st day to 16 th weeks after inection （P 〈 0.01）. Conclusion： Klebsiella Pneumoniae infection can cause lung varied ceils injury and increase c-fos protein and mRNA expression, and it play an important role in aggravating COPD.
出处
《天津医药》
CAS
北大核心
2005年第8期504-507,共4页
Tianjin Medical Journal
基金
江苏大学医学院博士科研启动基金资助项目(项目编号:9936)
