摘要
利用人血红蛋白(Hb)抗体制备葡萄球菌A蛋白协同凝聚试验(SPA)菌体试剂,对不同浓度人Hb稀释液进行反应,结果表明,以1%SPA免疫试剂浓度效果最佳,其最适Hb反应浓度在1~100mg/L当人Hb浓度超过1.5g/L时,免疫反应则出现假阴性结果(前带现象)。为消除免疫反应中的前带现象,作者对Beaglo犬粪上清液的作用效果进行了探讨。结果提示,1%免疫试剂浓度、5%粪液标本与2%Bea-glo犬粪上清液浓度为(SPA)免疫潜血试验最佳配对反应条件,将其用于3294名无症状人群普查,检出的5例大肠癌均呈阳性反应,因此具有推广应用价值。
By using SPA-test. in which specific antibody to human hemoglobin was coated with protein A contained staphylococcus, human Hb was tested in different dilutions. It was showed that 1% SPA reagent was effective to detect Hb in concentration of 1-- 100mg/L. Immunological negative response (zone phenomenon) appeared if concentration of Hb exceeded 1.5g/L. It was also showed that zone phenomenon could be diminished by the addition of a fecal suspension of Beaglo dog. The negative results for 1.5, 3. 10g/L Hb solution in SPA test might turn to be positive if 2% fecal suspension of Beaglo dog wasadded. 1% SPA reagent. 5% fecal sample together with 2% fecal suspension of dog therefore afforded the optimal condition for IFOBT. By using these quantitative ratio of SPA test. 5 colorectal cancer were identified in 3294 asymptomatic persons in a mass screening. No malignency was missed.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
1994年第2期92-94,共3页
Medical Journal of Chinese People's Liberation Army
关键词
大肠癌
SPA免疫
便潜血试验
HB
SPA
菌体试剂
Colorectal careinorna
Mass screening
Immunological fecal occult blood test
Zone phenomenon
SPA-test