摘要
目的探讨急性胰腺炎(AP)时花生四烯酸代谢产物血栓素(TXB2)和前列环素(PGI2)的变化及钙拮抗剂异搏定的治疗作用。方法采用十二指肠闭袢法诱发AP大鼠模型,设定AP组和异搏定治疗组,观察2组血浆TXB2、6酮基前列环素1α(6-keto-PGF1α)及其TXB2/6-keto-PGF1α比值的变化,同期行胰腺组织光镜和电镜的检查。结果诱发AP16h和24h,AP组血浆TXB2[(1112±235)pg/ml、(1265±162)pg/ml]和TXB2/6-keto-PGF1α比值[(9·9±0·9)、(10·2±1·3)]均较对照组[(282±56)pg/ml、(315±13)pg/ml,(5·4±2·2)、(5·7±1·8)]明显升高,治疗组血浆TXB2[(671±102)pg/ml、(697±93)pg/ml]及TXB2/6-keto-PGF1α比值[(6·9±2·2)、(6·4±0·7)]较AP组明显降低,同时胰腺组织病理损害程度明显减轻(P<0·05),且花生四烯酸代谢紊乱与胰腺病理损害程度呈正相关(P<0·05)。结论钙拮抗剂异搏定可改善大鼠AP时花生四烯酸代谢紊乱,减轻胰腺的病理损害。
Objective To evaluate the alterations of thromboxane （ TXB2 ） 、 prostacyclin （ PGI2 ） in murine acute pancreatitis （AP） and the therapeutic effect of calcium channel bocker-verapamil. Methods A rat AP model was established to observe the alterations of plasma levels of TXB2, 6-keto-PGF1α and TXB2/6-keto-PGF1α ratio and the effect of verapamil, Pancreatic histology was examined by light and electron microscopy. Results At 16 and 24 hours after induction of AP, the plasma levels of TXB2 [ （ 1112 ± 235 ） pg/ml、 （ 1265 ± 162 ） pg/ml ] and TXB2/6-keto-PGF1α ratio （ 9. 9 ± 0. 9, 10. 2 ± 1.3 ）increased significantly, With varapamil therapy, the plasma levels of TXB2 [ （ 671 ± 102 ） pg/ml, （ 697 ± 93 ） pg/ml] and TXB2/6-keto-PGF1α ratio （ 6.9 ± 2. 2 ）, （ 6.4 ± 0. 7 ） were dramatically lower than those in control group （ P 〈 0. 05 ）, the pathologic damages of pancreas was less severe （ P 〈 0. 05 ）. There was a positive relationship between eicosanoid imbalance and the severity of the pathology. Conclusion Verapamil decreases the plasma levels of TXB2 and stabilize TXB2/6-keto-PGF1α ratio andameliorates the pancreas pathologic damages.
出处
《中华普通外科杂志》
CSCD
北大核心
2005年第7期432-434,共3页
Chinese Journal of General Surgery
