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人类白细胞抗原-G cDNA的克隆及序列分析

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摘要 目的:构建表达人类白细胞抗原-G(HLA-G)蛋白的真核表达载体pcDNA3-HLA-G。方法:从孕妇绒毛组织中提取总RNA,借助RT-PCR技术扩增HLA-G的cDNA并把其插入真核表达载体pcDNA3,然后经酶切和测序法鉴定。结果:经酶切鉴定及测序分析,证实已构建表达HLA-G蛋白的真核表达载体pcDNA3-HLA-G。结论:应用RT-PCR扩增及DNA连接技术成功构建HLA-G的真核表达载体。
出处 《广西医科大学学报》 CAS 北大核心 2005年第3期370-372,共3页 Journal of Guangxi Medical University
基金 广西科技厅科技攻关项目(No.桂科攻0322025-21)
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