摘要
用离体小血管分析了大鼠基底动脉（ＢＡ）和肾动脉（ＲＡ）血管内皮及平滑肌上血管加压素受体亚型的分布，及与之耦联的平滑肌细胞钙动员之特征。结果表明，精氨酸血管加压素（ＡＶＰ）对内皮完整和去内皮之ＢＡ和ＲＡ均有强烈的收缩作用，后者可被Ｖ＿１受体选择性拮抗剂ｄ（ＣＨ＿２）＿５Ｔｙｒ（Ｍｅ）ＡＶＰ所拮抗。未观察到ＡＶＰ对ＢＡ和ＲＡ的内皮依赖性舒张作用。提示在ＢＡ和ＲＡ之平滑肌细胞上均有Ｖ＿１受体分布，介导ＡＶＰ的直接缩血管作用，这在ＡＶＰ对ＢＡ和ＲＡ的作用中占主导地位。在Ｖ＿１受体所耦联的平滑肌细胞钙动员方面，ＢＡ和ＲＡ有所不同：ＢＡ的ＡＶＰ敏感钙池明显小于ＲＡ，ＢＡ的收缩对胞外钙内流的依赖性较之ＲＡ大。硝苯吡啶不仅抑制ＡＶＰ诱发的Ｃａ￣（２＋）内流，而且可明显抑制胞内钙池的释放。ＢＡ对硝苯吡啶比ＲＡ敏感。蛋白激酶Ｃ参与了ＡＶＰ对ＢＡ和ＲＡ的钙动员和收缩作用。
he distribution of V_1 vasopressin receptor and the coupling Ca￣2+)
mobilization werestudied on isolated rat basilar(BA)and renal arteries(R).The results showed
that argininevasopressin(AVP ) could potently constrict BA and RA in both with and without
endothel-um conditions. The constrictive effect of AVP on BA and RA could be antagonized by
V_1 re-ceptor antagonist d(CH_2)_5Tyr (Me )AVP in a dose-dependent manner. AVP failed to
induceendothelium-dependent relaxatio in BA as ifi RA. The results demonstrated that the
con-strictive effect mediated by V_1 receptor which distributed in the smooth muscles was
predom-inant in the action of AVP on BA and RA. The capacity of AVp-sensitive Ca￣(2+)pool
was sig-nificantly smaller in BA than in RA. Thus ,the AVp-induced constriction was more
depen-dent on extracellular Ca￣(2+)in BA than in RA. Nifedipine(10￣(-5)mol/L)inhibitted not
only theAVP-induced Ca￣(2+) influx but also the AVp-induced intracellular Ca￣(2+) release,and
BA wasmore sensitive to nifedipine than RA. Protein kinase C was involved in AVp-induced
con-strictions in BA and RA.
出处
《基础医学与临床》
CSCD
1995年第5期43-48,共6页
Basic and Clinical Medicine
关键词
血管加压素受体
钙动员
基底动脉
肾动脉
大鼠
vasopressin vasopressin receptor Ca￣(2+)
mobilization vascular smcothmuscle basilar artery renal artery
