摘要
本文报道了用电穿孔方法将质粒ＤＮＡ导入人膀胱癌细胞的方法及效率，并对电穿孔过程中各参数与转化克隆形成率、细胞存活率以及某些与细胞转染有关的因素进行了研究与分析。
n this experiment we studied the method and its
efficiency of transducing pliasmid DNA in-to human bladder carcinoma cell line using
electroporation in vitro ,and described the relationshipamong electroporation parameters
,transfection efficiency and ceellular survival rate after electrro-poration. We also discussed
some other questions correlating with electroporation efficiency.
出处
《基础医学与临床》
CSCD
1995年第4期42-44,41,共4页
Basic and Clinical Medicine
关键词
电穿孔
基因转移
质粒DNA
人膀胱癌细胞
癌细胞
plasmid NDA human
bladder carcinoma cell line electroporation Institute of Basic Medical Sciences ,Chinese
Academy of Medical Science,100005
