摘要
用０．５ｍｏｌ／Ｌ二氯二甲基硅烷（ＤＤｓｉ）－１．５ｍｏｌ／Ｌ苯酚／ＣＤＭ－ＨＯＡｃ（１：１）溶液代替经典的８５％三氟醋酸（ＴＦＡ）／Ｈ２Ｏ溶液对肽－Ｗａｎｇ树脂进行酸解释放游离肽反应。测定了裂解前后树脂上肽的取代度并分析了游离肽产物的氨基酸组成，证明裂解收率及产物纯度与ＴＦＡ法相同。ＤＤＳｉ法的成本仅相当于ＴＦＡ法的４．３％。
Dichlorodimethylsilane (DDSi)- phenol complex has been tested as cleaving reagent for the peptidyl Wang-resin linkage. Four model peptides were released from their Wang-resin linked precursors under the conditions of 0.5mol/L DDSi-1.5mol/L phenol/DCM-HOAc (l:1)/3% EDT/60min with the yield of 89. 2%～96. 5%, which was quite comparable with that of conventional TFA cleavage. Reasonable purity of all peptides was confirmed by the analysis of amino acid composition and HPLC profile. Two of them were characterized by FAB-MS also.
出处
《化学试剂》
CAS
CSCD
北大核心
1995年第2期101-103,共3页
Chemical Reagents
基金
国家自然科学基金
